Precise documentation of every single oocyte.

Every oocyte is unique. Several methods have been developed to evaluate the development potential on cell and cleavage level. Oocytes are extracted, undergo a treatment, are frozen if necessary, transported, stored, thawed, cultivated again and transferred. Enough reasons to create documentation on oocyte level. The EU Directive "Cells and Tissue" also requires documentation on identifiability and traceability of each oocyte.

Every single obtained oocyte will be represented visually in a graphical overview. It allows entries for a maximum of ten cultivation days, although more then 5 to 6 days will most likely never be necessary. In the overview, development stages and manipulations such as insemination and injections, assisted hatching, polar body diagnosis, freezing and thawing processes, embryo transfer and more, can be assigned exact to the cell and day. All this can be done with a few mouse-clicks on the corresponding cells.

Before entering data you should consider if you wish to put the focus of your documentation

• on summarized entries,
• or also on the individual oocyte.

In the first case you should enter the data only in the table view, while in the second you should use the graphical representation. The graphical representation can also be used to "fine-tune” entries made in the tabular view.

The table and graphical representations are linked logically. Changes made in one of the two views will also be represented in the other.

Treatments and treatment semen analysis

	Press this button to start the thawing process.
	With this button you can go to the cryo storage and freeze the wished number of oocytes.
	Reason for cancelation before ET. Change the options for reason for no ET in the system tables in the settings.
	Treatment(s) and semen analysis: Double-click to see treatment semen analysis
	To unlock a view, click on the text in the lower part of the screen
	Data input is now possible and the text changes

 

 

Table representation

This table allows you to efficiently and quickly enter all culture types in a DIR-compliant way. You can document aspiration cycles (IVF/ICSI), thawing cycles, conservation processes and alle possible combinations of these three.

	Advantages: Fast
	Disadvantages: Documentation of sums only, not on cell level

 

Options of cryopreservation

You have three options (without the cryopreservation button) to freeze oocytes from the culture:

	If you enter the number of oocytes, PNs or embryos, the program will transfer it automaticaly in the correct field of the cryo conservation window. Example: 2 PN selection --> Cryo field #PN IVF
	You can also go to the cryo storage, if you double click on the number of oocytes for cryo in the table view.
	If you click on an empty cryo field, the oocyte / embryo cryopreservation will be opened. Now you can enter the number of oocytes at their state.

 

Read more about the cultivation of oocytes with the table overview in the how to.

 

Graphical representation

The graphical representation allows a documentation of each oocyte, its development status and oocyte-specific processes on all culture days.

If that appears too complex, you can quickly insert data within a table overview. There you only need to enter summarized information about obtained, treated, fertilized and transferred oocyte.

The table and graphical representations are linked logically. Changes made in one of the two views will also be represented in the other.

You can document a maximum of 10 culture days. MedITEX IVF add oocytes to the matrix that have been obtain by follicle aspiration or thawing.

	Advantages: You can document each individual oocyte on all culture days; you can enter assisted hatching and polar body diagnostics; complient to EU directive
	Disadvantages: More time-consuming in the case of a large number of oocytes.

 

Duplicate / delete treatment

	If you use the oocytes at different treatments (IVF and ICSI), two semen analysis are required. You have to add them first at the tabele view.
	At the graphical view (if the data input possible) you can also duplicate semen analysis if you will use different samples for oocytes.
	Here you can see the duplicate result (SPIII).
	Now you can assign the third treatment to a PN or embryo development line. The current used semen analysis is bold marked.

 

 

Photo view

	In Photo view mode, imported images are displayed when available. To import images, double-click the desired cell and click on Add file from the new window.
	If Photo view is not active, a small camera icon will indicate that an image is available for viewing.

For both modes, you can view an enlarged image by moving your move over the cell.

 

 

Editing oocytes

Within the matrix you have two basic options to edit the oocytes:

1. Double-click to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics.
2. Right-click to enter treatments, stages and modifications.

 

 

Select several oocytes

Two options for selecting several oocytes at the same time:

You can use this functionality only for one day (one column). Edit the oocytes of every day separately.

This functionality is a considerable time-saving if you froze oocytes or give all (a lot of) oocytes the same state.

	1. Press the Ctrl key and select the wished oocytes of one day.
	2. Click the first oocyte, press the shift key and click at the last wished oocyte. Every oocyte between both will be selected too.

 

 

States of oocytes

	Discarded oocyte: Discarded oocytes are marked with a red cross in the matrix.
	Frozen oocyte: Those that are to bei frozen are marked with a snowflake symbol.
	Selected for transfer: Pronucleus stages that are selected for an embryo transfer are marked with a green circle.
	Transfered embryo: Those that are to be trasfered are marked with the symbol "ET".
	PBD conducted: Oocytes that have been subject to a polar body diagnosis are marked with a probe symbol.
	Assisted hatching conducted: Embryos subject to assisted hatching are marked with a laser symbol.

 

Before entering data you should consider if you wish to put the focus of your documentation only on D.I.R. compliance or also on the individual oocytes. Enter the data accordingly, either in the tabular or graphical representation. Attention: If you switch for working back in the table overview, and activate the data input, the detail informations about every oocyte were lost. Enter all culture days per oocyte from left to right. Always indicate the development stage first, then add a modification if needed.

Read more about the cultivation of oocytes with the graphical overview in the how to.

 

 

Summed representation

This representation serves as an overview of all processes during the cell culture.

An additional summed representation provides a quick overview of the culture processes. The tabs allow you to change between the views with a single mouse click.

Read more about the using in the how to.

 

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