How to cultivate oocytes with the Graphical representation tab?

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The graphical view allows you to enter data exact to the day. Furthermore, all cultivated oocytes and the modifications on them can be documented visually.

  • You can document a maximum of 10 culture days.
  • MedITEX IVF adds oocytes cells to the matrix that have been obtain by follicle aspiration or thawing.                    

 

Within the matrix you have two basic options to edit the oocytes:

  1. Double-click to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics.
  2. Right-click to enter treatments, stages and modifications.

 

Double-click on the oocyte 

A new window will open in which you can enter different information:

graph10.png

Click the gray shaded triangle to select a development stage from the list.

Alternatively, you can enter the first letter(s) in the field “Stage” and simply select the entry you are looking for.

If needed, enter further data such as a score or note.

After pressing the button “Add picture”, you can add a random number of pictures to the oocyte. You can magnify a picture by double-clicking it.

If "Assisted hatching" or/and a "polar body diagnosis" or/and a "PGD" have been or will be conducted, you have to press the corresponding tabs and tick the boxes. Then enter the respective values for AH, PBD or PGD. You can also add the used incubator.

If assisted hatching or a PBD have already been conducted with an oocyte, the information will be used for the next oocyte selected (after a query by the system).

Confirm your entries by clicking "OK“. In this example you can see the following in the matrix:

All entries can be edited again by double-clicking on the oocyte.

 

Right-click on the oocyte

After right-clicking the oocyte, a context menu will open, in which you can select the modifications and the development stage for the oocyte.

All changes that are made here will be represented in the table view! Therefore, check if fields have a red background again and correct your entries accordingly.

After choosing an IVF or ICSI treatment under the menu-item "Oocyte treatments", this will be displayed in the upper part of the "Culture" window.

Cul3.png

Double-click on the corresponding treatment. The window "Treatment semen analysis" will open. Click here, to go to the window.

 

The IVF treatment, for example, will be displayed in the matrix as follows:

If you wish to treat further cells on the same day, you do not have to assign the treatment spermiogram again.

 

States of oocytes

Discarded oocyte:

Discarded oocytes are marked with a red cross in the matrix.

Frozen oocyte:

Those that are to bei frozen are marked with a snowflake symbol.

Selected for transfer:

Pronucleus stages that are selected for an embryo transfer are marked with a green circle.                                       

Transfered embryo:

Those that are to be trasfered are marked with the symbol "ET".

PBD conducted:

Oocytes that have been subject to a polar body diagnosis are marked with a probe symbol.

Assisted hatching conducted:

Embryos subject to assisted hatching are marked with a laser symbol.

Enter all culture days per oocyte from left to right. Always indicate the development stage first, then add a modification if needed.

 

In case of an IVF, the graphical representation of a transfered oocyte would look as follows:

 

In case of an ICSI, the scheme could look like this:

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