How to cultivate oocytes with the Graphical representation tab?

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<p>The graphical view allows you to <strong>enter data exact to the day</strong>. Furthermore, all cultivated oocytes and the modifications on them can be documented visually.</p>
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<p>The <strong>graphical representation</strong> allows the documentation of individual<strong> oocytes/embryos</strong>, its development status and oocyte-specific processes on all culture days.</p>
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<table border="0">
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<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<td>
 
<td>
 
<ul>
 
<ul>
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<li>You can document a maximum of 10 culture days.</li>
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<li><strong>MedITEX IVF</strong> adds oocytes/embryos obtained by <strong>follicle aspiration</strong> or <strong>thawing</strong> procedures.</li>
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<li>
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<li>You can document the development up to 10 days.</li>
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<p>MedITEX IVF adds oocytes cells to the matrix that have been obtain by follicle aspiration or thawing.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
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</li>
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</ul>
 
</ul>
 
</td>
 
</td>
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</table>
 
</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p>Within the matrix you have three basic options to edit the oocytes/embryos:</p>
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<p>Within this view, you have three options to edit the oocytes/embryos:</p>
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<table border="0">
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<table style="margin-left: auto; margin-right: auto;" border="0" width="319" height="106">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/graph1.png" alt="" /></td>
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<td colspan="2"><ol>
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<td><ol>
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<li>Edit one or more fields with the <a href="/index.php?title=Cycle_Culture#1._Right-panel."><strong>right-panel</strong></a>.<strong> <br /></strong></li>
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<li><strong>Edit one or more fields</strong> with the <strong>vertical grid</strong><strong>&nbsp;</strong></li>
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<li><a href="/index.php?title=Cycle_Culture#2._Double-click"><strong>Double-click</strong></a> to enter development <strong>stages</strong>, <strong>scores</strong>, <strong>notes</strong>, <strong>photos</strong>,<strong> assisted hatching</strong> and <strong>polar body diagnostics</strong>. </li>
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<li><strong>Double-click</strong> to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics. </li>
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<li><a href="/index.php?title=Cycle_Culture#3._Right-click"><strong>Right-click</strong></a> to enter <strong>treatments</strong>, <strong>stages </strong>and <strong>modifications</strong>.</li>
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<li><strong>Right-click</strong> to enter treatments, stages and modifications.</li>
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</ol></td>
 
</ol></td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o0.png" alt="" /></td>
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<td><strong>Oocytes/embryos</strong> added to the <strong>Graphical representation</strong> view, will initially display the following icon.<br /></td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<h3>Edit one or more fields with the vertical grid</h3>
 
<h3>Edit one or more fields with the vertical grid</h3>
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<table border="0">
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<p>This is the quickest way of entering data. You can see all existing data of the <strong>selected oocyte/embryo</strong> and editing this information is simply performed by changing the related fields.<br />To edit the information for oocytes/embryos, select a cell from the <strong>Graphical representation </strong>view and add the relevant data to the <strong>Right-panel</strong>.</p>
 +
<p>You can also edit multiple oocytes/embryos at the same time. Simply select several of them holding the <strong>CTRL</strong> or <strong>Shift </strong>keys  (they must be in the same culture day). Keep in mind that the  information added will be the same for all selected oocytes/embryos.</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>This is the<strong> quickest input option</strong> in this area. You can <strong>see all existing data</strong> of the <strong>selected oocyte / embryo</strong>.<br />Every data, which you can enter in the next two options, you can also enter in the grid at the right side.<br /><strong>Already entered</strong> data are <strong>visible </strong>in the right table.</p>
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<p>&nbsp;</p>
 
<p>Read more about the possibilities to <strong>select several fields of one day</strong> in the next section <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos"><strong>Select several oocytes/embryos</strong></a>.</p>
 
<p>Read more about the possibilities to <strong>select several fields of one day</strong> in the next section <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos"><strong>Select several oocytes/embryos</strong></a>.</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p>&nbsp;</p>
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<h3>Double-click on the oocyte<strong>/embryo</strong></h3>
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<h3>Double-click on the oocyte<strong>&nbsp;</strong></h3>
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<p>A new window will open in which you can enter required data:</p>
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<p>A new window will open in which you can enter different information:</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<table border="0">
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<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<td>[[Image:graph10.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/graph10.png| ]]</td>
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<td><img src="/images/graph10.png" alt="" width="692" height="716" /><br /></td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>Click the <strong>gray shaded triangle</strong> to <strong>select a development stage </strong>from the list.</p>
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<p>&nbsp;</p>
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<table border="0">
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<p>The <strong>Add file</strong> functionality lets you insert as many pictures as you need. Additionally, once an image is added, you can magnify it by <strong>double-clicking </strong>it.</p>
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<tbody>
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<p>The tabs <strong>Assisted hatching</strong>, <strong>Polar body diagnosis </strong>and <strong>PGD</strong> have to be selected if those procedures were conducted. In the tabs, check the box for the corresponding procedures and enter remaining information.</p>
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<tr>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<td>
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<p>Alternatively, you can enter the first letter(s) in the field <strong>&ldquo;Stage&rdquo;</strong> and simply select the entry you are looking for.</p>
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</td>
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−
</tr>
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−
</tbody>
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−
</table>
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<p>If needed, enter further data such as a score or note.</p>
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<p>After pressing the button<strong> &ldquo;Add picture&rdquo;</strong>, you can add a random number of pictures to the oocyte. You can magnify a picture by double-clicking it.</p>
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<p>If<strong> "Assisted hatching"</strong> or/and a <strong>"polar body diagnosis"</strong> or/and a <strong>"PGD"</strong> have been or will be conducted, you have to press the corresponding tabs and tick the boxes. Then enter the respective values for AH, PBD or PGD. You can also add the used incubator.</p>
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<table border="0">
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<tbody>
 
<tbody>
 
<tr>
 
<tr>
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</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>If assisted hatching or a PBD have already been conducted with an oocyte, the information will be used for the next oocyte selected (after a query by the system).</p>
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<p>&nbsp;</p>
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<p>Confirm your entries by clicking <strong>"OK&ldquo;</strong>. In this example you can see the following in the matrix:</p>
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<p>Confirm your entries by clicking <strong>OK</strong> and the appearance of the oocyte/embryo will be changed.</p>
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<table border="0">
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<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<td><img src="/images/graph5.png" alt="" width="200" height="65" /></td>
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<td><img src="/images/graph5.png" alt="" /></td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>All entries can be edited again by double-clicking on the oocyte.</p>
 
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<h3>Right-click on the oocyte</h3>
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<h3>Right-click on the oocyte/embryo</h3>
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<p>After right-clicking the oocyte, a context menu will open, in which you can select the modifications and the development stage for the oocyte.</p>
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<p>After <strong>right-clicking</strong> the oocyte/embryo, a menu will open in   which you can select the changes on the development stage.</p>
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<table border="0">
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<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
 
<td><img src="/images/graph6.png" alt="" /></td>
 
<td><img src="/images/graph6.png" alt="" /></td>
 
<td>
 
<td>
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<p>All changes that are made here will be represented in the table view! Therefore, check if fields have a red background again and correct your entries accordingly.</p>
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<p><strong>All changes</strong> that are made here will be represented in the <strong>Table representation</strong> as well.</p>
 +
<p>If you change the input method to the <strong>Table representation</strong> tab, the stages will not be recorded anymore.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>After choosing an IVF or ICSI treatment under the menu-item<strong> "Oocyte treatments"</strong>, this will be displayed in the upper part of the <strong>"Culture"</strong> window.</p>
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<p>&nbsp;</p>
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<table border="0">
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<h3>States of oocytes</h3>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
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<td>[[Image:Cul3.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/Cul3.png| ]]</td>
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<td><img src="/images/o0.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Unknown</strong>:</p>
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<p>Oocytes that did not have their stage selected.</p>
 +
</td>
 
</tr>
 
</tr>
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</tbody>
 
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</table>
 
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<p>Double-click on the corresponding treatment. The window <strong>"Treatment semen analysis"</strong> will open. Click <a href="/index.php?title=Treatments_and_treatment_semen_analysis">here</a>, to go to the window.</p>
 
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<p>&nbsp;</p>
 
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<p>The <strong>IVF treatment</strong>, for example, will be displayed in the matrix as follows:</p>
 
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<table border="0">
 
−
<tbody>
 
 
<tr>
 
<tr>
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<td><img src="/images/graph7.png" alt="" width="70" height="40" /></td>
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<td><img src="/images/o1.png" alt="" /></td>
 +
<td>
 +
<p><strong>Discarded</strong>:</p>
 +
<p>Discarded oocytes are marked with a red cross.</p>
 +
</td>
 
</tr>
 
</tr>
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</tbody>
 
−
</table>
 
−
<table border="0">
 
−
<tbody>
 
 
<tr>
 
<tr>
−
<td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<td><img src="/images/o2.png" alt="" /></td>
 
<td>
 
<td>
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<p>If you wish to treat further cells on the same day, you do not have to assign the treatment spermiogram again.</p>
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<p><strong>Frozen</strong>:<strong><br /></strong></p>
 +
<p style="text-align: justify;">Frozen oocytes are marked with a snowflake symbol.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
−
</tbody>
 
−
</table>
 
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<p>&nbsp;</p>
 
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<h3>States of oocytes</h3>
 
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<table border="0">
 
−
<tbody>
 
 
<tr>
 
<tr>
−
<td><img src="/images/o1.png" alt="" width="70" height="52" /></td>
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<td><img src="/images/o3.png" alt="" /></td>
 
<td>
 
<td>
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<p><strong>Discarded oocyte:</strong></p>
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<p><strong>Selected for transfer</strong>:</p>
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<p>Discarded oocytes are marked with a red cross in the matrix.</p>
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<p style="text-align: justify;">Pro-nucleus      stages that are selected for an embryo transfer are marked with a     green  circle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img src="/images/o2.png" alt="" width="70" height="49" /></td>
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<td><img src="/images/o4.png" alt="" /></td>
 
<td>
 
<td>
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<p><strong>Frozen oocyte:</strong></p>
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<p><strong>Transferred</strong>:</p>
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<p>Those that are to bei frozen are marked with a snowflake symbol.</p>
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<p>Those that are to be transferred are marked with the&nbsp;<strong>ET</strong> symbol.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img src="/images/o3.png" alt="" width="70" height="47" /></td>
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<td><img src="/images/o7.png" alt="" /></td>
 
<td>
 
<td>
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<p><strong>Selected for transfer:</strong></p>
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<p><strong>In arrest</strong>:<strong>&nbsp;</strong></p>
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<p>Pronucleus stages that are selected for an embryo transfer are marked  with a green circle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
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<p>Oocytes that stopped developing.<strong><br /></strong></p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img src="/images/o4.png" alt="" width="70" height="49" /></td>
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<td><img src="/images/o5.png" alt="" /></td>
 
<td>
 
<td>
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<p><strong>Transfered embryo:</strong></p>
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<p><strong>PBD</strong>:</p>
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<p>Those that are to be trasfered are marked with the symbol "ET".</p>
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<p style="text-align: justify;">Oocytes that have been subjected to a polar body diagnosis.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img src="/images/o5.png" alt="" width="70" height="49" /></td>
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<td><img src="/images/o10.png" alt="" width="55" height="38" /></td>
 
<td>
 
<td>
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<p><strong>PBD conducted:</strong></p>
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<p><strong>PGD</strong>:</p>
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<p>Oocytes that have been subject to a polar body diagnosis are marked with a probe symbol.</p>
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<p>Oocytes that have been subjected to a pre-implantation genetic diagnosis.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img src="/images/o6.png" alt="" width="70" height="51" /></td>
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<td><img src="/images/o6.png" alt="" /></td>
 
<td>
 
<td>
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<p><strong>Assisted hatching conducted:</strong></p>
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<p><strong>Assisted hatching</strong>:</p>
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<p>Embryos subject to assisted hatching are marked with a laser symbol.</p>
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<p>Embryos subjected to assisted hatching.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<td><img src="/images/o9.png" alt="" width="55" height="38" /></td>
 
<td>
 
<td>
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<p>Enter all culture days per oocyte <strong>from left to right</strong>. Always indicate the development stage first, then add a modification if needed.</p>
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<p><strong>IVF</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o8.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>ICSI</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
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</table>
 
</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p>In case of an IVF, the graphical representation of a transfered oocyte would look as follows:</p>
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<h3>Treatment semen analysis</h3>
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<table border="0">
+
<p><strong>Double-click</strong> on the corresponding semen analysis. The window <strong>Treatment semen analysis</strong> will open.</p>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td><img src="/images/graph8.png" alt="" /></td>
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<td>[[Image:Cul3.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/Cul3.png| ]]</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p>In case of an ICSI, the scheme could look like this:</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
−
<table border="0">
+
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td><img src="/images/graph9.png" alt="" /></td>
+
<td><img src="/images/plus48.png" alt="" width="48" height="48" /></td>
 +
<td><a href="/index.php?title=Treatments_and_treatment_semen_analysis">Click here</a> to have more information about the <strong>Treatment semen analysis</strong> window.</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>

Latest revision as of 11:32, 15 April 2013

The graphical representation allows the documentation of individual oocytes/embryos, its development status and oocyte-specific processes on all culture days.

<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />
  • MedITEX IVF adds oocytes/embryos obtained by follicle aspiration or thawing procedures.
  • You can document the development up to 10 days.

 

Within this view, you have three options to edit the oocytes/embryos:

<tbody> </tbody>
  1. Edit one or more fields with the <a href="/index.php?title=Cycle_Culture#1._Right-panel.">right-panel</a>.
  2. <a href="/index.php?title=Cycle_Culture#2._Double-click">Double-click</a> to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics.
  3. <a href="/index.php?title=Cycle_Culture#3._Right-click">Right-click</a> to enter treatments, stages and modifications.
<img src="/images/o0.png" alt="" /> Oocytes/embryos added to the Graphical representation view, will initially display the following icon.

 

Contents

Edit one or more fields with the vertical grid

This is the quickest way of entering data. You can see all existing data of the selected oocyte/embryo and editing this information is simply performed by changing the related fields.
To edit the information for oocytes/embryos, select a cell from the Graphical representation view and add the relevant data to the Right-panel.

You can also edit multiple oocytes/embryos at the same time. Simply select several of them holding the CTRL or Shift keys (they must be in the same culture day). Keep in mind that the information added will be the same for all selected oocytes/embryos.

<tbody> </tbody>
<img src="/images/vg1.png" alt="" width="765" />

 

Read more about the possibilities to select several fields of one day in the next section <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">Select several oocytes/embryos</a>.

 

Double-click on the oocyte/embryo

A new window will open in which you can enter required data:

<tbody> </tbody>
<img src="/images/graph10.png" alt="" width="692" height="716" />

 

The Add file functionality lets you insert as many pictures as you need. Additionally, once an image is added, you can magnify it by double-clicking it.

The tabs Assisted hatching, Polar body diagnosis and PGD have to be selected if those procedures were conducted. In the tabs, check the box for the corresponding procedures and enter remaining information.

<tbody> </tbody>
<img src="/images/graph3.png" alt="" /> <img src="/images/graph4.png" alt="" />

 

Confirm your entries by clicking OK and the appearance of the oocyte/embryo will be changed.

<tbody> </tbody>
<img src="/images/graph5.png" alt="" />

 

Right-click on the oocyte/embryo

After right-clicking the oocyte/embryo, a menu will open in which you can select the changes on the development stage.

<tbody> </tbody>
<img src="/images/graph6.png" alt="" />

All changes that are made here will be represented in the Table representation as well.

If you change the input method to the Table representation tab, the stages will not be recorded anymore.

 

States of oocytes

<tbody> </tbody>
<img src="/images/o0.png" alt="" width="55" height="38" />

Unknown:

Oocytes that did not have their stage selected.
<img src="/images/o1.png" alt="" />

Discarded:

Discarded oocytes are marked with a red cross.
<img src="/images/o2.png" alt="" />

Frozen:

Frozen oocytes are marked with a snowflake symbol.
<img src="/images/o3.png" alt="" />

Selected for transfer:

Pro-nucleus stages that are selected for an embryo transfer are marked with a green circle.                                       
<img src="/images/o4.png" alt="" />

Transferred:

Those that are to be transferred are marked with the ET symbol.
<img src="/images/o7.png" alt="" />

In arrest: 

Oocytes that stopped developing.
<img src="/images/o5.png" alt="" />

PBD:

Oocytes that have been subjected to a polar body diagnosis.
<img src="/images/o10.png" alt="" width="55" height="38" />

PGD:

Oocytes that have been subjected to a pre-implantation genetic diagnosis.
<img src="/images/o6.png" alt="" />

Assisted hatching:

Embryos subjected to assisted hatching.
<img src="/images/o9.png" alt="" width="55" height="38" />

IVF:

Indicates the insemination method used.
<img src="/images/o8.png" alt="" width="55" height="38" />

ICSI:

Indicates the insemination method used.

 

Treatment semen analysis

Double-click on the corresponding semen analysis. The window Treatment semen analysis will open.

<tbody> </tbody>
Cul3.png

 

<tbody> </tbody>
<img src="/images/plus48.png" alt="" width="48" height="48" /> <a href="/index.php?title=Treatments_and_treatment_semen_analysis">Click here</a> to have more information about the Treatment semen analysis window.
<tbody> </tbody>
<a href="/index.php?title=MedITEX_IVF_how_to">Back to How to</a> <a href="#top">Back to top</a>