How to cultivate oocytes with the Graphical representation tab?

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<p>&nbsp;</p>
 
<p>&nbsp;</p>
 
<h3>States of oocytes</h3>
 
<h3>States of oocytes</h3>
−
<table border="0">
+
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td><img src="/images/o1.png" alt="" width="70" height="52" /></td>
+
<td><img src="/images/o0.png" alt="" width="55" height="38" /></td>
 
<td>
 
<td>
−
<p><strong>Discarded oocyte:</strong></p>
+
<p><strong>Unknown</strong>:</p>
−
<p>Discarded oocytes are marked with a red cross in the matrix.</p>
+
<p>Oocytes that did not have their stage selected.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img src="/images/o2.png" alt="" width="70" height="49" /></td>
+
<td><img src="/images/o1.png" alt="" /></td>
 
<td>
 
<td>
−
<p><strong>Frozen oocyte:</strong></p>
+
<p><strong>Discarded</strong>:</p>
−
<p>Those that are to bei frozen are marked with a snowflake symbol.</p>
+
<p>Discarded oocytes are marked with a red cross.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img src="/images/o3.png" alt="" width="70" height="47" /></td>
+
<td><img src="/images/o2.png" alt="" /></td>
 
<td>
 
<td>
−
<p><strong>Selected for transfer:</strong></p>
+
<p><strong>Frozen</strong>:<strong><br /></strong></p>
−
<p>Pronucleus stages that are selected for an embryo transfer are marked with a green circle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
+
<p style="text-align: justify;">Frozen oocytes are marked with a snowflake symbol.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img src="/images/o4.png" alt="" width="70" height="49" /></td>
+
<td><img src="/images/o3.png" alt="" /></td>
 
<td>
 
<td>
−
<p><strong>Transfered embryo:</strong></p>
+
<p><strong>Selected for transfer</strong>:</p>
−
<p>Those that are to be trasfered are marked with the symbol "ET".</p>
+
<p style="text-align: justify;">Pro-nucleus    stages that are selected for an embryo transfer are marked with a  green  circle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img src="/images/o5.png" alt="" width="70" height="49" /></td>
+
<td><img src="/images/o4.png" alt="" /></td>
 
<td>
 
<td>
−
<p><strong>PBD conducted:</strong></p>
+
<p><strong>Transferred</strong>:</p>
−
<p>Oocytes that have been subject to a polar body diagnosis are marked with a probe symbol.</p>
+
<p>Those that are to be trasferred are marked with the&nbsp;<strong>ET</strong> symbol.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img src="/images/o6.png" alt="" width="70" height="51" /></td>
+
<td><img src="/images/o7.png" alt="" /></td>
 
<td>
 
<td>
−
<p><strong>Assisted hatching conducted:</strong></p>
+
<p><strong>In arrest</strong>:<strong>&nbsp;</strong></p>
−
<p>Embryos subject to assisted hatching are marked with a laser symbol.</p>
+
<p>Oocytes that stopped developing.<strong><br /></strong></p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/hint48.png" alt="" width="48" height="48" /></td>
+
<td><img src="/images/o5.png" alt="" /></td>
 
<td>
 
<td>
−
<p>Enter all culture days per oocyte <strong>from left to right</strong>. Always indicate the development stage first, then add a modification if needed.</p>
+
<p><strong>PBD</strong>:</p>
 +
<p style="text-align: justify;">Oocytes that have been subjected to a polar body diagnosis.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o10.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>PGD</strong>:</p>
 +
<p>Oocytes that have been subjected to a pre-implantation genetic diagnosis.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o6.png" alt="" /></td>
 +
<td>
 +
<p><strong>Assisted hatching</strong>:</p>
 +
<p>Embryos subjected to assisted hatching.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o9.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>IVF</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o8.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>ICSI</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>

Revision as of 12:14, 8 April 2013

The graphical view allows you to enter data exact to the day. Furthermore, all cultivated oocytes and the modifications on them can be documented visually.

<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />
  • You can document a maximum of 10 culture days.

  • MedITEX IVF adds oocytes/embryos to the matrix that have been obtain by follicle aspiration or thawing.                    

 

Within the matrix you have three basic options to edit the oocytes/embryos:

<tbody> </tbody>
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/graph1.png" alt="" />
  1. Edit one or more fields with the vertical grid 
  2. Double-click to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics.
  3. Right-click to enter treatments, stages and modifications.

 

Contents

Edit one or more fields with the vertical grid

<tbody> </tbody>
<img src="/images/vg1.png" alt="" width="765" />

This is the quickest input option in this area. You can see all existing data of the selected oocyte / embryo.
Every data, which you can enter in the next two options, you can also enter in the grid at the right side.
Already entered data are visible in the right table.

Read more about the possibilities to select several fields of one day in the next section <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">Select several oocytes/embryos</a>.

 

 

Double-click on the oocyte/embryo

A new window will open in which you can enter different information:

<tbody> </tbody>
graph10.png

Click the gray shaded triangle to select a development stage from the list.

<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />

Alternatively, you can enter the first letter(s) in the field “Stage” and simply select the entry you are looking for.

If needed, enter further data such as a score or note.

After pressing the button “Add picture”, you can add a random number of pictures to the oocyte/embryo. You can magnify a picture by double-clicking it.

If "Assisted hatching" or/and a "polar body diagnosis" or/and a "Preimplantation genetic diagnosis" have been or will be conducted, you have to press the corresponding tabs and tick the boxes. Then enter the respective values for AH, PBD or PGD. You can also add the used incubator.

<tbody> </tbody>
<img src="/images/graph3.png" alt="" /> <img src="/images/graph4.png" alt="" />

If assisted hatching, PBD or PGD have already been conducted with an oocyte/embryo, the information will be used for the next oocyte/embryo selected (after a query by the system).

Confirm your entries by clicking "OK“. In this example you can see the following in the matrix:

<tbody> </tbody>
<img src="/images/graph5.png" alt="" width="200" height="65" />

All entries can be edited again by double-clicking on the oocyte/embryo.

 

Right-click on the oocyte/embryo

After right-clicking the oocyte/embryo, a context menu will open, in which you can select the modifications and the development stage for the oocyte/embryo.

<tbody> </tbody>
<img src="/images/graph6.png" alt="" />

All changes that are made here will be represented in the table view! Therefore, check if fields have a red background again and correct your entries accordingly.

After choosing an IVF or ICSI treatment under the menu-item "Oocyte treatments", this will be displayed in the upper part of the "Culture" window.

<tbody> </tbody>
Cul3.png

Double-click on the corresponding treatment. The window "Treatment semen analysis" will open. Click <a href="/index.php?title=Treatments_and_treatment_semen_analysis">here</a>, to go to the window.

 

The IVF treatment, for example, will be displayed in the matrix as follows:

<tbody> </tbody>
<img src="/images/graph7.png" alt="" width="70" height="40" />
<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />

If you wish to treat further oocytes on the same day, you do not have to assign the treatment spermiogram again.

 

States of oocytes

<tbody> </tbody>
<img src="/images/o0.png" alt="" width="55" height="38" />

Unknown:

Oocytes that did not have their stage selected.
<img src="/images/o1.png" alt="" />

Discarded:

Discarded oocytes are marked with a red cross.
<img src="/images/o2.png" alt="" />

Frozen:

Frozen oocytes are marked with a snowflake symbol.
<img src="/images/o3.png" alt="" />

Selected for transfer:

Pro-nucleus stages that are selected for an embryo transfer are marked with a green circle.                                       
<img src="/images/o4.png" alt="" />

Transferred:

Those that are to be trasferred are marked with the ET symbol.
<img src="/images/o7.png" alt="" />

In arrest: 

Oocytes that stopped developing.
<img src="/images/o5.png" alt="" />

PBD:

Oocytes that have been subjected to a polar body diagnosis.
<img src="/images/o10.png" alt="" width="55" height="38" />

PGD:

Oocytes that have been subjected to a pre-implantation genetic diagnosis.
<img src="/images/o6.png" alt="" />

Assisted hatching:

Embryos subjected to assisted hatching.
<img src="/images/o9.png" alt="" width="55" height="38" />

IVF:

Indicates the insemination method used.
<img src="/images/o8.png" alt="" width="55" height="38" />

ICSI:

Indicates the insemination method used.

 

In case of an IVF, the graphical representation of a transfered oocyte would look as follows:

<tbody> </tbody>
<img src="/images/graph8.png" alt="" />

 

In case of an ICSI, the scheme could look like this:

<tbody> </tbody>
<img src="/images/graph9.png" alt="" />
<tbody> </tbody>
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