Cycle Culture
From MedITEX - Wiki
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<p><strong>Selected for transfer:</strong></p> | <p><strong>Selected for transfer:</strong></p> | ||
| − | <p>Pronucleus stages that are selected for an embryo transfer are marked with a green circle.</p> | + | <p>Pronucleus stages that are selected for an embryo transfer are marked with a green circle. </p> |
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Revision as of 17:26, 25 February 2013
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Precise documentation of every single oocyte.
Every oocyte is unique. Several methods have been developed to evaluate the development potential on cell and cleavage level. Oocytes are extracted, undergo a treatment, are frozen if necessary, transported, stored, thawed, cultivated again and transferred. Enough reasons to create documentation on oocyte level. The EU Directive "Cells and Tissue" also requires documentation on identifiability and traceability of each oocyte.
MedITEX IVF allows detailed documentation of all development stages and manipulations of oocyte – exact to the day, including photos, scores, comments, performed analysis etc.
MedITEX IVF for the first time allows you to document complex laboratory processes realistically, for example post maturing of individual oocyte, adding of thawed oocytes or performing fertilization on the following day.
Every single obtained oocyte will be represented visually in a graphical overview. It allows entries for a maximum of ten cultivation days, although more then 5 to 6 days will most likely never be necessary. In the overview, development stages and manipulations such as insemination and injections, assisted hatching, polar body diagnosis, freezing and thawing processes, embryo transfer and more, can be assigned exact to the cell and day. All this can be done with a few mouse-clicks on the corresponding cells.
Contents |
Before entering data you should consider if you wish to put the focus of your documentation
- on summarized entries,
- or also on the individual oocyte.
In the first case you should enter the data only in the table view, while in the second you should use the graphical representation. The graphical representation can also be used to "fine-tune” entries made in the tabular view.
The table and graphical representations are linked logically. Changes made in one of the two views will also be represented in the other.
Table representation
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This table allows you to efficiently and quickly enter all culture types in a DIR-compliant way. You can document aspiration cycles (IVF/ICSI), thawing cycles, conservation processes and alle possible combinations of these three.
| Advantages: | Fast, DIR-compliant |
| Disadvantages: |
Documentation of sums only, not on cell level |
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| <a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" width="139" height="35" /></a> | Press this button to start the thawing process. |
| <a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/Cryopre.png" alt="" width="137" height="31" /></a> |
With this button you can go to the cryo storage and freeze the wished number of oocytes. |
| <a href="/index.php?title=Cryo_storage"><img src="/images/cryocon.png" alt="" width="100" height="125" /></a> |
You can also go to the cryo storage, if you double click on the number of oocytes for cryo in the table view. |
| <a href="/index.php?title=Freezing_a_new_sample"><img src="/images/cryocon1.png" alt="" width="100" height="126" /></a> | If you click on an empty cryo field, the oocyte / embryo cryopreservation will be opened. |
| <img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" /> | Reason for cancelation before ET |
| <a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/cryoprov2.png" alt="" width="481" height="86" /></a> |
Treatment(s) and semen analysis: Double-click to see treatment semen analysis |
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<img style="float: left;" src="/images/datainput2.png" alt="" width="356" height="15" /> |
To unlock a view, click on the text in the lower part of the screen |
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<img style="float: left;" src="/images/datainput.png" alt="" width="207" height="15" /> |
Data input is now possible and the text changes |
Graphical representation
The graphical representation allows a documentation of each oocyte, its development status and oocyte-specific processes on all culture days.
If that appears too complex, you can quickly insert data within a table overview. There you only need to enter summarized information about obtained, treated, fertilized and transferred oocyte.
The table and graphical representations are linked logically. Changes made in one of the two views will also be represented in the other.
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You can document a maximum of 10 culture days. MedITEX IVF add oocytes to the matrix that have been obtain by follicle aspiration or thawing.
| Advantages: |
You can document each individual oocyte on all culture days; you can enter assisted hatching and polar body diagnostics; complient to EU. Directive; compliant to DIR. |
| Disadvantages: |
More time-consuming in the case of a large number of oocytes. |
Within the matrix you have two basic options to edit the oocytes:
- Double-click to enter development stages, scores, notes, photos, assisted hatching and polar body diagnostics.
- Right-click to enter treatments, stages and modifications.
| <img src="/images/hint48.png" alt="" width="48" height="48" /> |
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| <img src="/images/plus48.png" alt="" width="48" height="48" /> | Click here, to get more informations about entering informations. |
| States of oocytes: | |
| <img src="/images/o1.png" alt="" width="114" height="84" /> |
Discarded oocyte: Discarded oocytes are marked with a red cross in the matrix. |
| <img src="/images/o2.png" alt="" width="114" height="80" /> |
Frozen oocyte: Those that are to bei frozen are marked with a snowflake symbol. |
| <img src="/images/o3.png" alt="" width="113" height="76" /> |
Selected for transfer: Pronucleus stages that are selected for an embryo transfer are marked with a green circle. |
| <img src="/images/o4.png" alt="" width="114" height="79" /> |
Transfered embryo: Those that are to be trasfered are marked with the symbol "ET". |
| <img src="/images/o5.png" alt="" width="112" height="79" /> |
PBD conducted: Oocytes that have been subject to a polar body diagnosis are marked with a probe symbol. |
| <img src="/images/o6.png" alt="" width="113" height="82" /> |
Assisted hatching conducted: Embryos subject to assisted hatching are marked with a laser symbol. |
| <img src="/images/hint48.png" alt="" width="48" height="48" /> |
Enter all culture days per oocyte from left to right. Always indicate the development stage first, then add a modification if needed. |
Summed representation
This representation serves as an overview of all processes during the cell culture.
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An additional summed representation provides a quick overview of the culture processes. The tabs allow you to change between the views with a single mouse click.
| <img src="/images/howto48.png" alt="" width="48" height="48" /> | Read more about the using in the <a href="/index.php?title=MedITEX_IVF_how_to#Cycle">how to</a>. |
| <a href="/index.php?title=MedITEX_IVF_manual">Back to the MedITEX IVF menu</a><a href="/index.php?title=Cycles"></a> | <a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=Cycles">Back to the cycle overview</a> | <a href="#top">Back to top</a> |