Cycle Culture

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<td>[[Image:CultureTable.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CultureTable.png| ]]</td>
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<td style="text-align: center;"><a href="/index.php?title=Cycle_Cryo"><img src="/images/plus48.png" alt="" width="48" height="48" /></a></td>
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<td><a href="/index.php?title=Cycle_Cryo">Click here</a> for <strong>Cryo/Donation</strong> cycle.</td>
 
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<p><strong>Precise documentation of every single oocyte.</strong></p>
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<p>Every oocyte is <strong>unique</strong>.<strong> Several methods</strong> have been developed to  evaluate the development potential on cell and cleavage level. Oocytes are  <strong>extracted</strong>, <strong>undergo a treatment</strong>, are <strong>frozen </strong>if necessary, <strong>transported</strong>, <strong> stored</strong>, <strong>thawed</strong>, <strong>cultivated again</strong> and <strong>transferred</strong>.    Enough reasons to  create documentation on oocyte level. The EU  Directive  "Cells and Tissue"  also requires documentation on  identifiability and  traceability of each  oocyte.</p>
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<p>MedITEX IVF allows <strong>detailed documentation</strong> of <strong>all development stages</strong> and <strong>manipulations</strong> of oocyte &ndash; <strong>exact to the day</strong>, including <strong>photos</strong>, <strong> scores</strong>, <strong>comments</strong>, <strong>performed analysis</strong> etc.</p>
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<p>MedITEX IVF for the first time allows you to document complex    laboratory processes realistically, for example post maturing of    individual oocyte, adding of thawed oocytes or performing    fertilization on the following day.</p>
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<p>Every single obtained oocyte will be represented <strong>visually </strong>in a <strong> graphical </strong>overview. It allows entries for a <strong>maximum of ten cultivation  days</strong>, although <strong>more then 5 to 6 days</strong> will most likely <strong>never </strong>be  necessary. In the overview, <strong>development stages</strong> and <strong>manipulations </strong>such as  <strong>insemination </strong>and <strong>injections</strong>, <strong>assisted hatching</strong>, <strong>polar body diagnosis</strong>, <strong> freezing </strong>and <strong>thawing processes</strong>, <strong>embryo transfer</strong> and <strong>more</strong>, can be  assigned <strong>exact to the cell and day</strong>. All this can be done with a few  mouse-clicks on the corresponding cells.</p>
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<p>[[Image:ct1.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/ct1.png| ]]</p>
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<p>&nbsp;</p>
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<p style="text-align: justify;">Every single oocyte/embryo can be documented in <strong>MedITEX IVF</strong>. The tab <strong>Culture </strong>allows entries for a maximum of ten cultivation  days, although more than 5 to 6 days will most likely never be  necessary. In the overview, <strong>development stages</strong> and <strong>manipulations </strong>such as  <strong>insemination </strong>and <strong>injections</strong>, <strong>assisted hatching</strong>, <strong>polar body diagnosis</strong>, <strong> freezing </strong>and <strong>thawing processes</strong>, <strong>embryo transfer</strong> and <strong>more</strong>, can be  assigned to a specific date and stage.</p>
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<p>__TOC__</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<p><strong>Before entering data</strong> you should consider if you wish to put the focus      of your <strong>documentation </strong></p>
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<p style="text-align: justify;">Before entering data, you should consider the focus      of you documentation.</p>
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<p style="text-align: justify;"><strong>MedITEX IVF</strong> provides two possibilities:</p>
 
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<li><strong>on summarized entries</strong>, </li>
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<li>A more simplified and summarized documentation, called <strong>Table representation</strong>; </li>
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<li>or also <strong>on the  individual  oocyte</strong>. </li>
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<li>And a more complete version &ndash; <strong>Graphical representation</strong>. </li>
 
</ul>
 
</ul>
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<p>In the <strong>first case</strong> you should enter the data only  in  the <strong> table   view</strong>, while in the <strong>second </strong>you should use the <strong>graphical     representation</strong>.  The graphical representation can also be used to <strong> "fine-tune&rdquo; </strong>entries  made in the tabular view.</p>
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<p style="text-align: justify;">The <strong>table </strong>and <strong>graphical representations</strong> are linked logically. Therefore, changes     made in one of the two views will also be represented in the other.</p>
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<p>The table and graphical representations are <strong>linked logically</strong>. Changes     made in one of the two views will also be represented in the other.</p>
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<p style="text-align: justify;">&nbsp;</p>
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<h3>Table representation</h3>
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<td>[[Image:TableD.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/TableD.png| ]]</td>
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<td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<td>
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<p>You can only input data in one of the representations. If you are documenting oocytes/embryos in <strong>Table representation</strong>, <strong>Graphical representation</strong> will be locked.</p>
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<p>You can lock/unlock these sections by clicking on the <strong><span style="color: #ff0000;">red </span></strong>or <span style="color: #339966;"><strong>green </strong></span>text at the bottom of this screen.</p>
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<p>This table allows you to <strong>efficiently and quickly</strong> enter all  culture types in a DIR-compliant way. You can document <strong>aspiration  cycles</strong> (IVF/ICSI), <strong>thawing cycles</strong>, <strong>conservation processes</strong> and <strong>alle  possible combinations</strong> of these three.</p>
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<p>&nbsp;</p>
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<table border="0">
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<h2>Treatments and treatment semen analysis</h2>
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<td><strong>Advantages:</strong></td>
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<td>Fast, DIR-compliant</td>
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<p>[[Image:CulTr.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CulTr.png| ]]</p>
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<p>&nbsp;</p>
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<td><strong>Disadvantages</strong>:&nbsp; <br /></td>
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<td style="text-align: left;"><a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" /></a></td>
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<td>Documentation of sums only, not on cell level</td>
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<td>Starts the thawing process.</td>
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<td style="text-align: center;"><a href="/index.php?title=Cryo_storage"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/Cryopre.png" alt="" /></a></td>
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<td>
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<p style="text-align: justify;">Opens cryo storage so you can freeze oocytes/embryos.</p>
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</td>
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<tr>
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<td style="text-align: center;"><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" /></td>
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<td>
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<p>Documents the reason why there was no embryo transfer for this cycle.<strong>&nbsp;</strong></p>
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<p>You can edit the options available here at the <a href="/index.php?title=MedITEX_IVF_Settings#System_tables">system tables</a> in the settings.<br /><br /></p>
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</td>
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</tr>
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<tr>
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<td style="text-align: center;"><a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/cryoprov2.png" alt="" width="400" height="72" /></a></td>
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<td>
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<p><strong>Treatments and semen analysis</strong>:</p>
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<p style="text-align: justify;"><strong>Double-click</strong> to see <strong>treatment and semen analysis</strong>. You do not need a <strong>semen analysis</strong> for <strong>thawed cycles</strong>.</p>
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<br /></td>
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</tr>
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<td style="text-align: center;"><img src="/images/datainput2.png" alt="" width="356" height="15" /></td>
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<td>This indicates that either the <strong>Table </strong>or <strong>Graphical representation</strong> are locked and cannot be changed. Click the text to unlock.</td>
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<td style="text-align: center;"><img src="/images/datainput.png" alt="" width="207" height="15" /></td>
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<td>Indicates that this section is unlocked and can be edited.<br /></td>
 
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<table border="0">
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<h2 style="text-align: left;">Table representation</h2>
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<p>This table allows you to <strong>efficiently and quickly</strong> document all types of cycles: <strong>aspiration </strong>(IVF/ICSI), <strong>thawing</strong>, etc.</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
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<td>[[Image:crypov.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/crypov.png| ]]</td>
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<td colspan="2">[[Image:TableD.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/TableD.png| ]]</td>
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</tr>
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<tr>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<td>
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<p><strong>Advanta</strong><strong>ges</strong>: faster and simpler.</p>
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<p><strong>Disadvantages</strong>: documentation of sums only, not on a cell level.</p>
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</td>
 
</tr>
 
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</tbody>
 
</tbody>
 
</table>
 
</table>
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<p>&nbsp;</p>
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<h3 style="text-align: justify;">Options for cryopreservation</h3>
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<p style="text-align: justify;">From the <strong>Culture tab</strong>, you have three options for freezing oocytes/embryos:</p>
 
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<td style="text-align: center;"><a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" width="139" height="35" /></a></td>
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<td style="text-align: center;"><img src="/images/ov2.png" alt="" width="450" height="272" /></td>
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<td>Press this button to <strong>start the thawing process</strong>.<br /></td>
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<td style="text-align: justify;">
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<p><strong>1. </strong>If you enter the <strong>number of oocytes</strong>, <strong>PNs</strong> or <strong>embryos</strong>, the program will <strong>automatically transfer it</strong> to the <strong>correct field</strong> of the <strong>cryo preservation</strong> window.</p>
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</td>
 
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<tr>
 
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<td style="text-align: center;"><a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/Cryopre.png" alt="" width="137" height="31" /></a></td>
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<td style="text-align: center;"><a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/cryocon.png" alt="" /></a><br /></td>
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<td style="text-align: justify;"><strong>2. </strong>You can also open the <a href="/index.php?title=Cryo_storage"><strong>cryo storage</strong></a>, if you <strong>double-click </strong>the number of oocytes for <strong>Cryo</strong> in the <strong>table view</strong>.</td>
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<tr>
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<td style="text-align: center;">&nbsp;<a href="/index.php?title=Freezing_a_new_sample"><img style="border: 2px dotted blue;" src="/images/cryocon1.png" alt="" /></a></td>
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<td style="text-align: justify;"><strong>3. </strong>If you click on an <strong>empty cryo field</strong>, the <strong>oocyte/embryo cryopreservation</strong> will be opened. Now you can enter the number of oocytes/embryos to cryopreserve.</td>
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</tr>
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<p>&nbsp;</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<tbody>
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<tr>
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<td style="text-align: center;"><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Table_representation_tab%3F"><img src="/images/howto48.png" alt="" /></a></td>
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<td><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Table_representation_tab%3F">Click here</a> to read more about the cultivation of oocytes/embryos with the <strong>Table representation</strong> view. <br /></td>
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</tr>
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</tbody>
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</table>
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<p>&nbsp;</p>
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<h2 style="text-align: left;">Graphical representation</h2>
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<p style="text-align: justify;">The <strong>graphical representation</strong> allows the documentation of individual<strong> oocytes/embryos</strong>, its development status and oocyte-specific processes on all culture days.</p>
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<p style="text-align: justify;">The <strong>table </strong>and <strong>graphical representations </strong>are logically linked<strong>&nbsp;</strong>.  Meaning that, changes    made in one of the views will also be  represented in the other. Keep in mind that you can only make changes  into one representation at a time and, to switch the representation  method, you need to unlock the desired one by clicking the <span style="color: #ff0000;"><strong>red </strong></span>text at the bottom of the window.</p>
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<p style="text-align: justify;">After  performing the follicle aspiration procedure, the same exact amount of  follicles will be available for cultivation here on the <strong>Graphical representation</strong>. Ten days are available for you to document the growth progress.</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<tbody>
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<td colspan="2">[[Image:CulG.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CulG.png| ]]</td>
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</tr>
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<tr>
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<td style="text-align: center;"><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
 
<td>
 
<td>
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<p>With this button you can <strong>go to the cryo storage</strong> and freeze the wished number of oocytes.</p>
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<p><strong>Advantages</strong>: you can document progress on each  individual oocyte/embryo on all culture days; you can    enter assisted  hatching, polar body diagnostics, pre-implantation genetic diagnosis and  used incubators.</p>
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<p><strong>Disadvantages</strong>: may be time-consuming in case of a large number of oocytes/embryos. Click <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">here</a> to learn how to select multiple cells and <a href="/index.php?title=Cycle_Culture#Editing_oocytes_.2F_embryos">here</a> to learn how to edit multiple cells.</p>
 
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<p>&nbsp;</p>
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<h3>Duplicate / delete treatment</h3>
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<table style="margin-left: auto; margin-right: auto;" border="0" width="691" height="826">
 
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cryocon.png" alt="" width="100" height="125" /></td>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul8.png" alt="" /></td>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cryocon1.png" alt="" width="100" height="126" /></td>
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<td>
 
<td>
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<p>You can also go to the cryo storage, if you <strong>double click on the number of oocytes</strong> for cryo in the <strong>table view</strong>.</p>
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<p>If you use oocytes/embryos for different treatments (<strong>IVF </strong>and <strong>ICSI</strong>), two semen analysis are required.</p>
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<p>Then, add them at the <strong>Table representation</strong> view.</p>
 
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul9.png" alt="" /></td>
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<td>At the <strong>Graphical representation</strong>, you can also <strong>duplicate </strong>semen analysis if you plan to use different samples for oocytes/embryos.</td>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul10.png" alt="" /></td>
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<td>Here you can see the duplicate result<strong> - SPIII</strong>.</td>
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<td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul11.png" alt="" /></td>
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<p>If you click on an <strong>empty cryo field</strong>, the <strong>oocyte / embryo cryopreservation</strong> will be opened.</p>
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<p>Now you can assign it to a PN or embryo.</p>
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<p>The semen analysis with <strong>bold </strong>letters indicates that it is currently selected for that oocyte/embryo.</p>
 
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<h3>Select several oocytes / embryos</h3>
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<p style="text-align: justify;">There are two ways of selecting several oocytes/embryos at the same time.</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
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<ul>
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<li>You can use this functionality only for cells on the same day.</li>
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<li>This functionality saves you a lot of time<strong> </strong>if you plan to freeze or apply the same stage to several oocytes/embryos<strong>&nbsp;</strong>.</li>
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</ul>
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<p style="text-align: justify;"><strong><br /></strong></p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<tbody>
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<tr>
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<td><img src="/images/Oo2.png" alt="" /></td>
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<td style="text-align: justify;">Hold the <strong>Ctrl key</strong> and select multiple cells on the same day.</td>
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</tr>
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<tr>
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<td><img src="/images/Oo1.png" alt="" /></td>
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<td style="text-align: justify;"><ol>
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<li>Select&nbsp;the first cell and hold <strong>Shift</strong>;</li>
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<li>Click on the last desired cell;<strong>&nbsp;</strong></li>
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<li>All cells in between will be selected. </li>
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</ol></td>
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<h3>Editing oocytes / embryos</h3>
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<p>Within the matrix you have<strong> three options to edit </strong>the oocytes/embryos:</p>
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<h4><strong>1.</strong> <strong>Right-panel</strong>.</h4>
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<p>This is the quickest way of entering data. You can see all existing data of the <strong>selected oocyte/embryo</strong> and editing this information is simply performed by changing the related fields.<br />To edit the information for oocytes/embryos, select a cell from the <strong>Graphical representation </strong>view and add the relevant data to the <strong>Right-panel</strong>.</p>
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<p>You can also edit multiple oocytes/embryos at the same time. Simply select several of them holding the <strong>CTRL</strong> or <strong>Shift </strong>keys (they must be in the same culture day). Keep in mind that the information added will be the same for all selected oocytes/embryos.</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<td>[[Image:vg1.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/vg1.png| ]]</td>
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<td style="text-align: center;"><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" /></td>
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<td><a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos"><img src="/images/hint48.png" alt="" width="48" height="48" /></a></td>
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<td>&nbsp;</td>
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<td><a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">Click here</a> to read more about selecting several oocytes/embryos.</td>
 
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</tbody>
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</table>
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<p>&nbsp;</p>
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<h4><strong>2. Double-click</strong></h4>
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<p><strong>Double-click</strong> an oocyte/embryo to enter development stages, scores,  notes, photos, assisted hatching, pre-implantation genetic diagnosis,  polar body diagnostics and used incubators.</p>
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<p>A new window will open in which you can enter different information:</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<tr>
 
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<td><a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/TreatCul.png" alt="" width="592" height="86" /></a></td>
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<td><img src="/images/graph10.png" alt="" width="692" height="716" /><br /></td>
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<td>&nbsp;</td>
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</tr>
 
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</tbody>
 
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</table>
 
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
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<table style="margin-left: auto; margin-right: auto;" border="0">
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<tbody>
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<td><img src="/images/howto48.png" alt="" width="48" height="48" /></td>
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<td>Read more about the <strong>cultivation of oocytes with the graphical overview</strong> in the <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">how to</a>.</td>
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<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<h3 style="text-align: left;">Graphical representation</h3>
+
<h4><strong>3. Right-click</strong></h4>
−
<p>The graphical representation allows a documentation of each egg cell, its development status and cell-specific processes on all culture days.</p>
+
<p><strong>Right-click</strong> an oocyte/embryo to enter treatments, stages, incubators, etc.</p>
−
<table border="0">
+
<p>After <strong>right-clicking</strong> the oocyte/embryo, a <strong>context menu</strong> will open, in which you  can select the modifications and the development stage for the oocyte/embryo.</p>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td>[[Image:CultureM.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CultureM.png| ]]</td>
+
<td><img src="/images/graph6.png" alt="" /></td>
 +
<td>
 +
<p><strong>All changes</strong> that are made here will be represented in the<strong> Table representation </strong>view! However, check if fields have a <strong>red background</strong> and <strong>correct </strong>your  entries accordingly.</p>
 +
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
−
<p>If that appears too complex, you can quickly insert data within a    table overview. There you only need to enter summarized information    about obtained, treated, fertilized and transferred oocyte.</p>
+
<p>&nbsp;</p>
−
<p>The table and graphical representations are linked logically. Changes    made in one of the two views will also be represented in the other.</p>
+
<table style="margin-left: auto; margin-right: auto;" border="0">
−
<p>The graphical representation can also be used to "fine-tune&rdquo; entries made in the tabular view.</p>
+
−
<table border="0">
+
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td><strong>Advantages:</strong></td>
+
<td><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F"><img src="/images/howto48.png" alt="" width="48" height="48" /></a></td>
 +
<td><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the <strong>Graphical representation</strong> view.</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p>&nbsp;</p>
 +
<h3>States of oocytes</h3>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td><img src="/images/o0.png" alt="" width="55" height="38" /></td>
 
<td>
 
<td>
−
<p>You can document each individual oocyte on all culture days; you can enter assisted hatching and polar body diagnostics; complient to EU.&nbsp;Directive; compliant to DIR.</p>
+
<p><strong>Unknown</strong>:</p>
 +
<p>Oocytes that did not have their stage selected.</p>
 
</td>
 
</td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
−
<td><strong>Disadvantages</strong>:&nbsp; <br /></td>
+
<td><img src="/images/o1.png" alt="" /></td>
−
<td>More time-consuming in the case of a large number of oocytes.</td>
+
<td>
 +
<p><strong>Discarded</strong>:</p>
 +
<p>Discarded oocytes are marked with a red cross.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o2.png" alt="" /></td>
 +
<td>
 +
<p><strong>Frozen</strong>:<strong><br /></strong></p>
 +
<p style="text-align: justify;">Frozen oocytes are marked with a snowflake symbol.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/thaw.PNG" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Thawed:</strong></p>
 +
<p>Thawed oocytes are marked with a melting snowflake symbol.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o3.png" alt="" /></td>
 +
<td class="mceSelected" style="text-align: left;">
 +
<p><strong>Selected for transfer</strong>:</p>
 +
<p>Pro-nucleus  stages that are selected for an embryo transfer are marked with a  green  circle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/group.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Oocyte groups:</strong></p>
 +
<p>If there is any colored bar on the left, the oocyte is part of an embryo / oocyte group.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o4.png" alt="" /></td>
 +
<td>
 +
<p><strong>Transferred ideal</strong>:</p>
 +
<p>Those that are to be transferred are marked with the&nbsp;<strong>ET</strong> (green) symbol.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/et.PNG" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Transferred not ideal</strong> :</p>
 +
<p>Those that are to be transferred are marked with the&nbsp;<strong>ET</strong> (orange) symbol.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o7.png" alt="" /></td>
 +
<td>
 +
<p><strong>In arrest</strong>:<strong>&nbsp;</strong></p>
 +
<p>Oocytes that stopped developing.<strong><br /></strong></p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/Donation_Donated.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Donation - Donated:</strong></p>
 +
<p>Oocytes that have been donated are marked with an arrow right to the oocyte.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/Donation_received.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>Donation - Received:</strong></p>
 +
<p>Oocytes that have been received from a donation are marked with an arrow left to the oocyte.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o5.png" alt="" /></td>
 +
<td>
 +
<p><strong>PBD</strong>:</p>
 +
<p style="text-align: justify;">Oocytes that have been subjected to a polar body diagnosis.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o10.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>PGD</strong>:</p>
 +
<p>Oocytes that have been subjected to a pre-implantation genetic diagnosis.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o6.png" alt="" /></td>
 +
<td>
 +
<p><strong>Assisted hatching</strong>:</p>
 +
<p>Embryos subjected to assisted hatching.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o9.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>IVF</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/o8.png" alt="" width="55" height="38" /></td>
 +
<td>
 +
<p><strong>ICSI</strong>:</p>
 +
<p>Indicates the insemination method used.</p>
 +
</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
−
<p>To unlock a view, click on the following text in the lower part of the screen:</p>
+
<table style="margin-left: auto; margin-right: auto;" border="0" width="523" height="126">
−
<p><img src="/images/datainput2.png" alt="" width="356" height="15" /></p>
+
−
<p>Data input is now possible and the text changes:</p>
+
−
<p><img src="/images/datainput.png" alt="" width="207" height="15" /></p>
+
−
<p>&nbsp;</p>
+
−
<table border="0">
+
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
Line 133: Line 412:
 
<td>
 
<td>
 
<ul>
 
<ul>
−
<li>The cells entered in the input mask<strong> &ldquo;Follicle asp.&rdquo;</strong> and also thawed and  frozen cells will automatically be displayed in the table <strong>and</strong> graphical representation.</li>
+
<li style="text-align: justify;"><strong><span style="color: #ff0000;">Attention</span></strong><span style="color: #ff0000;">&nbsp;</span>: if you switch back to the <strong>Table representation </strong>view, the detailed information about  each oocyte will be  lost.</li>
−
<li>Before entering data you should consider if you wish to put the focus of your documentation only on D.I.R. compliance or also on the individual egg cells. Enter the data accordingly, either in the tabular <span style="text-decoration: underline;">or</span> graphical representation.</li>
+
</ul>
 +
<ul>
 +
<li style="text-align: justify;">Document the growth of oocytes/embryos from<strong> left to right</strong>. Always indicate the development stage first, then add a modification if needed.</li>
 
</ul>
 
</ul>
 
</td>
 
</td>
 +
</tr>
 +
<tr>
 +
<td><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F"><img src="/images/howto48.png" alt="" width="48" height="48" /></a></td>
 +
<td><a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the <strong>Graphical representation</strong> view.</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
−
<h3>Summed representation</h3>
+
<p>&nbsp;</p>
−
<p>This representation serves as an overview of all processes during the cell culture.</p>
+
<h3>Photo view</h3>
−
<table border="0">
+
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td>[[Image:CultureSum.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CultureSum.png| ]]</td>
+
<td><img src="/images/Oo3.png" alt="" /></td>
 +
<td>In <strong>Photo view</strong> mode, imported images are displayed when available. To import images, <strong>double-click</strong> the desired cell and click on <strong>Add file </strong>from the new window.</td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/Oo4.png" alt="" width="332" height="201" /></td>
 +
<td>If <strong>Photo view </strong>is not active, a small camera icon will indicate that an image is available for viewing.</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
−
<p>An additional summed representation provides a quick overview of the    culture processes. The tabs allow you to change between the views with  a  single mouse click.</p>
+
<p>&nbsp;</p>
−
<table border="0">
+
<table style="margin-left: auto; margin-right: auto;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td><img src="/images/howto48.png" alt="" width="48" height="48" /></td>
+
<td><img src="/images/Oo5.png" alt="" width="340" height="251" /></td>
−
<td>Read more about the using in the <a href="/index.php?title=MedITEX_IVF_how_to#Cycle">how to</a>.</td>
+
<td>For <strong>both modes</strong>, you can view an <strong>enlarged image</strong> by <strong>moving your mouse over</strong> the cell.</td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
 +
<h3 style="text-align: justify;">Screenshot functionality</h3>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td>
 +
<p>[[Image:screenshot3.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/screenshot3.png| ]]</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p>&nbsp;</p>
 +
<p>Click on the <strong>camera </strong>symbol to export the current representation into the <a href="/index.php?title=Notebook_/_screenshots"><strong>Notebook tool</strong></a>. The following window will open:</p>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td><img src="/images/screenshot1.png" alt="" width="650" height="442" /></td>
 +
</tr>
 +
<tr>
 +
<td><img src="/images/screenshot2.png" alt="" width="650" /></td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p style="text-align: justify;">If you do not close the notebook window and you take a <strong>second screenshot</strong>, it will automatically be added below the first one.</p>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td><a href="/index.php?title=Notebook_/_screenshots"><img src="/images/plus48.png" alt="" width="48" height="48" /></a></td>
 +
<td><a href="/index.php?title=Notebook_/_screenshots">Click here</a> to read more about the <strong>Notebook / screenshots</strong> tool.</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p>&nbsp;</p>
 +
<h2>Summed representation</h2>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td>[[Image:CultureSum.png|none|740px|thumb|left|link=http://wiki.meditex-software.com/images/CultureSum.png| ]]</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
<p style="text-align: justify;">&nbsp;</p>
 +
<p style="text-align: justify;">An additional tab &ndash; <strong>Summed representation</strong> &ndash;<strong> </strong>provides a <strong>quick overview</strong> of the    <strong>culture progress</strong>.</p>
 +
<p style="text-align: justify;">Data input is not possible here.</p>
 +
<p style="text-align: justify;">&nbsp;</p>
 +
<hr />
 +
<p>&nbsp;</p>
 +
<table style="margin-left: auto; margin-right: auto;" border="0">
 +
<tbody>
 +
<tr>
 +
<td><a href="/index.php?title=MedITEX_IVF_how_to#Cycle"><img src="/images/howto48.png" alt="" width="48" height="48" /></a></td>
 +
<td><a href="/index.php?title=MedITEX_IVF_how_to#Cycle">Click here</a> to read more about this section<a href="/index.php?title=MedITEX_IVF_how_to#Cycle"></a>.</td>
 +
</tr>
 +
</tbody>
 +
</table>
 
<table style="float: right;" border="0">
 
<table style="float: right;" border="0">
 
<tbody>
 
<tbody>
 
<tr>
 
<tr>
−
<td style="text-align: right;"><a href="/index.php?title=MedITEX_IVF_manual">Back to the MedITEX IVF menu</a><a href="/index.php?title=Cycles"></a></td>
+
<td style="text-align: right;"><a href="/index.php?title=MedITEX_IVF_manual">Back to the MedITEX IVF menu</a></td>
−
<td style="text-align: right;"><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=Cycles">Back to the cycle overview</a></td>
+
<td style="text-align: right;"><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=Cycles">Back to the cycles</a></td>
 
<td style="text-align: right;"><a href="#top">Back to top</a></td>
 
<td style="text-align: right;"><a href="#top">Back to top</a></td>
 
</tr>
 
</tr>
 
</tbody>
 
</tbody>
 
</table>
 
</table>

Latest revision as of 12:25, 8 July 2015

<tbody> </tbody>
<a href="/index.php?title=Cycle_Cryo"><img src="/images/plus48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=Cycle_Cryo">Click here</a> for Cryo/Donation cycle.
<tbody> </tbody>

ct1.png

 

Every single oocyte/embryo can be documented in MedITEX IVF. The tab Culture allows entries for a maximum of ten cultivation days, although more than 5 to 6 days will most likely never be necessary. In the overview, development stages and manipulations such as insemination and injections, assisted hatching, polar body diagnosis, freezing and thawing processes, embryo transfer and more, can be assigned to a specific date and stage.

Contents

 

Before entering data, you should consider the focus of you documentation.

MedITEX IVF provides two possibilities:

  • A more simplified and summarized documentation, called Table representation;
  • And a more complete version – Graphical representation.

The table and graphical representations are linked logically. Therefore, changes made in one of the two views will also be represented in the other.

 

<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />

You can only input data in one of the representations. If you are documenting oocytes/embryos in Table representation, Graphical representation will be locked.

You can lock/unlock these sections by clicking on the red or green text at the bottom of this screen.

 

Treatments and treatment semen analysis

<tbody> </tbody>

CulTr.png

 

<tbody> </tbody>
<a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" /></a> Starts the thawing process.
<a href="/index.php?title=Cryo_storage"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/Cryopre.png" alt="" /></a>

Opens cryo storage so you can freeze oocytes/embryos.
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" />

Documents the reason why there was no embryo transfer for this cycle. 

You can edit the options available here at the <a href="/index.php?title=MedITEX_IVF_Settings#System_tables">system tables</a> in the settings.
<a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/cryoprov2.png" alt="" width="400" height="72" /></a>

Treatments and semen analysis:

Double-click to see treatment and semen analysis. You do not need a semen analysis for thawed cycles.
<img src="/images/datainput2.png" alt="" width="356" height="15" /> This indicates that either the Table or Graphical representation are locked and cannot be changed. Click the text to unlock.
<img src="/images/datainput.png" alt="" width="207" height="15" /> Indicates that this section is unlocked and can be edited.

 

Table representation

This table allows you to efficiently and quickly document all types of cycles: aspiration (IVF/ICSI), thawing, etc.

<tbody> </tbody>
TableD.png
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/hint48.png" alt="" width="48" height="48" />

Advantages: faster and simpler.

Disadvantages: documentation of sums only, not on a cell level.

 

Options for cryopreservation

From the Culture tab, you have three options for freezing oocytes/embryos:

<tbody> </tbody>
<img src="/images/ov2.png" alt="" width="450" height="272" />

1. If you enter the number of oocytes, PNs or embryos, the program will automatically transfer it to the correct field of the cryo preservation window.
<a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/cryocon.png" alt="" /></a>
 2. You can also open the <a href="/index.php?title=Cryo_storage">cryo storage</a>, if you double-click the number of oocytes for Cryo in the table view.
 <a href="/index.php?title=Freezing_a_new_sample"><img style="border: 2px dotted blue;" src="/images/cryocon1.png" alt="" /></a> 3. If you click on an empty cryo field, the oocyte/embryo cryopreservation will be opened. Now you can enter the number of oocytes/embryos to cryopreserve.

 

<tbody> </tbody>
<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Table_representation_tab%3F"><img src="/images/howto48.png" alt="" /></a> <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Table_representation_tab%3F">Click here</a> to read more about the cultivation of oocytes/embryos with the Table representation view.

 

Graphical representation

The graphical representation allows the documentation of individual oocytes/embryos, its development status and oocyte-specific processes on all culture days.

The table and graphical representations are logically linked . Meaning that, changes made in one of the views will also be represented in the other. Keep in mind that you can only make changes into one representation at a time and, to switch the representation method, you need to unlock the desired one by clicking the red text at the bottom of the window.

After performing the follicle aspiration procedure, the same exact amount of follicles will be available for cultivation here on the Graphical representation. Ten days are available for you to document the growth progress.

<tbody> </tbody>
CulG.png
<img src="/images/hint48.png" alt="" width="48" height="48" />

Advantages: you can document progress on each individual oocyte/embryo on all culture days; you can enter assisted hatching, polar body diagnostics, pre-implantation genetic diagnosis and used incubators.

Disadvantages: may be time-consuming in case of a large number of oocytes/embryos. Click <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">here</a> to learn how to select multiple cells and <a href="/index.php?title=Cycle_Culture#Editing_oocytes_.2F_embryos">here</a> to learn how to edit multiple cells.

 

Duplicate / delete treatment

<tbody> </tbody>
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul8.png" alt="" />

If you use oocytes/embryos for different treatments (IVF and ICSI), two semen analysis are required.

Then, add them at the Table representation view.
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul9.png" alt="" />
At the Graphical representation, you can also duplicate semen analysis if you plan to use different samples for oocytes/embryos.
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul10.png" alt="" />
Here you can see the duplicate result - SPIII.
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/cul11.png" alt="" />

Now you can assign it to a PN or embryo.

The semen analysis with bold letters indicates that it is currently selected for that oocyte/embryo.

 

Select several oocytes / embryos

There are two ways of selecting several oocytes/embryos at the same time.

<tbody> </tbody>
<img src="/images/hint48.png" alt="" width="48" height="48" />
  • You can use this functionality only for cells on the same day.
  • This functionality saves you a lot of time if you plan to freeze or apply the same stage to several oocytes/embryos .


<tbody> </tbody>
<img src="/images/Oo2.png" alt="" /> Hold the Ctrl key and select multiple cells on the same day.
<img src="/images/Oo1.png" alt="" />
  1. Select the first cell and hold Shift;
  2. Click on the last desired cell; 
  3. All cells in between will be selected.

 

Editing oocytes / embryos

Within the matrix you have three options to edit the oocytes/embryos:

1. Right-panel.

This is the quickest way of entering data. You can see all existing data of the selected oocyte/embryo and editing this information is simply performed by changing the related fields.
To edit the information for oocytes/embryos, select a cell from the Graphical representation view and add the relevant data to the Right-panel.

You can also edit multiple oocytes/embryos at the same time. Simply select several of them holding the CTRL or Shift keys (they must be in the same culture day). Keep in mind that the information added will be the same for all selected oocytes/embryos.

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vg1.png

 

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<a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos"><img src="/images/hint48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">Click here</a> to read more about selecting several oocytes/embryos.

 

2. Double-click

Double-click an oocyte/embryo to enter development stages, scores, notes, photos, assisted hatching, pre-implantation genetic diagnosis, polar body diagnostics and used incubators.

A new window will open in which you can enter different information:

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<img src="/images/graph10.png" alt="" width="692" height="716" />

 

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<img src="/images/howto48.png" alt="" width="48" height="48" /> Read more about the cultivation of oocytes with the graphical overview in the <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">how to</a>.

 

3. Right-click

Right-click an oocyte/embryo to enter treatments, stages, incubators, etc.

After right-clicking the oocyte/embryo, a context menu will open, in which you can select the modifications and the development stage for the oocyte/embryo.

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<img src="/images/graph6.png" alt="" />

All changes that are made here will be represented in the Table representation view! However, check if fields have a red background and correct your entries accordingly.

 

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<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F"><img src="/images/howto48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the Graphical representation view.

 

States of oocytes

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<img src="/images/o0.png" alt="" width="55" height="38" />

Unknown:

Oocytes that did not have their stage selected.
<img src="/images/o1.png" alt="" />

Discarded:

Discarded oocytes are marked with a red cross.
<img src="/images/o2.png" alt="" />

Frozen:

Frozen oocytes are marked with a snowflake symbol.
<img src="/images/thaw.PNG" alt="" width="55" height="38" />

Thawed:

Thawed oocytes are marked with a melting snowflake symbol.
<img src="/images/o3.png" alt="" />

Selected for transfer:

Pro-nucleus stages that are selected for an embryo transfer are marked with a green circle.                                       
<img src="/images/group.png" alt="" width="55" height="38" />

Oocyte groups:

If there is any colored bar on the left, the oocyte is part of an embryo / oocyte group.
<img src="/images/o4.png" alt="" />

Transferred ideal:

Those that are to be transferred are marked with the ET (green) symbol.
<img src="/images/et.PNG" alt="" width="55" height="38" />

Transferred not ideal :

Those that are to be transferred are marked with the ET (orange) symbol.
<img src="/images/o7.png" alt="" />

In arrest: 

Oocytes that stopped developing.
<img src="/images/Donation_Donated.png" alt="" width="55" height="38" />

Donation - Donated:

Oocytes that have been donated are marked with an arrow right to the oocyte.
<img src="/images/Donation_received.png" alt="" width="55" height="38" />

Donation - Received:

Oocytes that have been received from a donation are marked with an arrow left to the oocyte.
<img src="/images/o5.png" alt="" />

PBD:

Oocytes that have been subjected to a polar body diagnosis.
<img src="/images/o10.png" alt="" width="55" height="38" />

PGD:

Oocytes that have been subjected to a pre-implantation genetic diagnosis.
<img src="/images/o6.png" alt="" />

Assisted hatching:

Embryos subjected to assisted hatching.
<img src="/images/o9.png" alt="" width="55" height="38" />

IVF:

Indicates the insemination method used.
<img src="/images/o8.png" alt="" width="55" height="38" />

ICSI:

Indicates the insemination method used.

 

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<img src="/images/hint48.png" alt="" width="48" height="48" />
  • Attention : if you switch back to the Table representation view, the detailed information about each oocyte will be lost.
  • Document the growth of oocytes/embryos from left to right. Always indicate the development stage first, then add a modification if needed.
<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F"><img src="/images/howto48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the Graphical representation view.

 

Photo view

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<img src="/images/Oo3.png" alt="" /> In Photo view mode, imported images are displayed when available. To import images, double-click the desired cell and click on Add file from the new window.
<img src="/images/Oo4.png" alt="" width="332" height="201" /> If Photo view is not active, a small camera icon will indicate that an image is available for viewing.

 

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<img src="/images/Oo5.png" alt="" width="340" height="251" /> For both modes, you can view an enlarged image by moving your mouse over the cell.

 

Screenshot functionality

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screenshot3.png

 

Click on the camera symbol to export the current representation into the <a href="/index.php?title=Notebook_/_screenshots">Notebook tool</a>. The following window will open:

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<img src="/images/screenshot1.png" alt="" width="650" height="442" />
<img src="/images/screenshot2.png" alt="" width="650" />

If you do not close the notebook window and you take a second screenshot, it will automatically be added below the first one.

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<a href="/index.php?title=Notebook_/_screenshots"><img src="/images/plus48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=Notebook_/_screenshots">Click here</a> to read more about the Notebook / screenshots tool.

 

Summed representation

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CultureSum.png

 

An additional tab – Summed representation – provides a quick overview of the culture progress.

Data input is not possible here.

 

 

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<a href="/index.php?title=MedITEX_IVF_how_to#Cycle"><img src="/images/howto48.png" alt="" width="48" height="48" /></a> <a href="/index.php?title=MedITEX_IVF_how_to#Cycle">Click here</a> to read more about this section<a href="/index.php?title=MedITEX_IVF_how_to#Cycle"></a>.
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