Coltura

Revision as of 14:04, 27 April 2016

<a href="/index.php?title=Cycle_Cryo">Cliccare qui</a> per Ciclo Solo Pick Up per Crioconservazione/Donazione.

Ogni singola cellula (ovocita/embrione) può essere documentata in MedITEX IVF. La tab Coltura permette di inserire informazioni per un massimo di 10 giorni di coltura, anche se nella maggior parte dei casi 5/6 giorni bastano. Nella overview è possibile assegnare per una specifica data e stadio della cellula informazioni riguradanti lo stadio di sviluppo e i trattamenti eseguiti, come per esempio l'inseminazione o iniezione, assisted hatching, diagnosi del globulo polare, crioconservazione e scongelamento, embrio transfer e molti altri.

Prima di cominciare ad inserire dati, sarebbe utile considerale lo scopo della documentazione.

MedITEX IVF fornisce due possibilità:

Una più semplice e riassuntiva forma di documentazione, chiamata Rappresentazione Tabellare;
e una più completa, chiamata Rappresentazione Grafica.

Le due rappresentazioni sono collegate in maniera logica, per cui cambiamenti riportati in una rappresentazione verranno automaticamente apportati anche all'altra.

L'inserimento di dati è possibile solamente in una rappresentazione. Se si inizia a documentare informazioni nella rappresentazione tabellare, quella grafica verrà bloccata.

Le due rappresentazioni possono essere bloccate/sbloccate cliccando sulla riga di testo rossa o verde sul fondo della schermo.

Sezione bloccata. L'input dei dati è possibile nell'altra rappresentazione.

Nel nostro esempio questa scritta è presente nella rappresentazione grafica e indca che è attiva quella tabellare. Per attivare la rappresentazione grafica premere sulla riga rossa, che si trasformerà in verde.

Sezione sbloccata e l'input dei dati è permesso.

Nel nostro esempio questa scritta è presente nella rappresentazone tabellare.

Passando da Rappresentazione Grafica a Tabellare alcune informazioni (stadio embrioni) già inserite potrebbero andare perse.

Trattamenti e Spermiogrammi

<a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" /></a>	Scongelare campioni.
<a href="/index.php?title=Cryo_storage"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/Cryopre.png" alt="" /></a>	Apre la criobanca permettendo la crioconservazione di ovociti/embrioni.
<img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" />	Campo riservato alla documentazione delle ragioni di mancato transfer.
<a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/cryoprov2.png" alt="" width="400" height="72" /></a>	Trattamenti e spermiogrammi: Doppio-click per visualizzare i trattamenti e gli spermiogrammi collegati alla terapia. Per cicli da scongelamento di zigoti/embrioni non è necessario collegare uno spermiogramma

Rappresentazione tabellare

Questa rappresentazione pernette di documentare in maniera efficiente e veloce tutti i tipi di ciclo: Con Pick Up (FIVET/ICSI), Scongelamento, etc.

Vantaggi: veloce e semplice.

Svantaggi: Documentazione sommaria, non a livello di singola cellula.

Opzioni per crioconservazione

Nella tab della Coltura, ci sono tre opzioni per la crioconservazione di ovociti/embrioni:

<img src="/images/ov2.png" alt="" width="450" height="272" />	1. Inserendo il numero si ovociti, PN o embrioni il programma inserirà automaticamente il numero nella sezione corretta nella finestra di crioconservazione.
<a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/cryocon.png" alt="" /></a>	2. Facendo doppio-click sul numero di cellule crioconservate dalla rappresentazione tabellare , è possibile aprire la criobanca. <a href="/index.php?title=Cryo_storage">cryo storage</a>
<a href="/index.php?title=Freezing_a_new_sample"><img style="border: 2px dotted blue;" src="/images/cryocon1.png" alt="" /></a>	3. If you click on an empty cryo field, the oocyte/embryo cryopreservation will be opened. Now you can enter the number of oocytes/embryos to cryopreserve.

<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Table_representation_tab%3F">Click here</a> to read more about the cultivation of oocytes/embryos with the Table representation view.

Rappresentazione grafica

The graphical representation allows the documentation of individual oocytes/embryos, its development status and oocyte-specific processes on all culture days.

The table and graphical representations are logically linked . Meaning that, changes made in one of the views will also be represented in the other. Keep in mind that you can only make changes into one representation at a time and, to switch the representation method, you need to unlock the desired one by clicking the red text at the bottom of the window.

After performing the follicle aspiration procedure, the same exact amount of follicles will be available for cultivation here on the Graphical representation. Ten days are available for you to document the growth progress.

Advantages: you can document progress on each individual oocyte/embryo on all culture days; you can enter assisted hatching, polar body diagnostics, pre-implantation genetic diagnosis and used incubators.

Disadvantages: may be time-consuming in case of a large number of oocytes/embryos. Click <a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">here</a> to learn how to select multiple cells and <a href="/index.php?title=Cycle_Culture#Editing_oocytes_.2F_embryos">here</a> to learn how to edit multiple cells.

Duplicate / delete treatment

If you use oocytes/embryos for different treatments (IVF and ICSI), two semen analysis are required.

Then, add them at the Table representation view.

At the Graphical representation, you can also duplicate semen analysis if you plan to use different samples for oocytes/embryos.

Here you can see the duplicate result - SPIII.

Now you can assign it to a PN or embryo.

The semen analysis with bold letters indicates that it is currently selected for that oocyte/embryo.

Select several oocytes / embryos

There are two ways of selecting several oocytes/embryos at the same time.

You can use this functionality only for cells on the same day.
This functionality saves you a lot of time if you plan to freeze or apply the same stage to several oocytes/embryos .

<img src="/images/Oo2.png" alt="" />	Hold the Ctrl key and select multiple cells on the same day.
<img src="/images/Oo1.png" alt="" />	Select the first cell and hold Shift; Click on the last desired cell; All cells in between will be selected.

Editing oocytes / embryos

Within the matrix you have three options to edit the oocytes/embryos:

1. Right-panel.

This is the quickest way of entering data. You can see all existing data of the selected oocyte/embryo and editing this information is simply performed by changing the related fields.
To edit the information for oocytes/embryos, select a cell from the Graphical representation view and add the relevant data to the Right-panel.

You can also edit multiple oocytes/embryos at the same time. Simply select several of them holding the CTRL or Shift keys (they must be in the same culture day). Keep in mind that the information added will be the same for all selected oocytes/embryos.

<a href="/index.php?title=Cycle_Culture#Select_several_oocytes_.2F_embryos">Click here</a> to read more about selecting several oocytes/embryos.

2. Double-click

Double-click an oocyte/embryo to enter development stages, scores, notes, photos, assisted hatching, pre-implantation genetic diagnosis, polar body diagnostics and used incubators.

A new window will open in which you can enter different information:

Read more about the cultivation of oocytes with the graphical overview in the <a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">how to</a>.

3. Right-click

Right-click an oocyte/embryo to enter treatments, stages, incubators, etc.

After right-clicking the oocyte/embryo, a context menu will open, in which you can select the modifications and the development stage for the oocyte/embryo.

All changes that are made here will be represented in the Table representation view! However, check if fields have a red background and correct your entries accordingly.

<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the Graphical representation view.

States of oocytes

<img src="/images/o0.png" alt="" width="55" height="38" />	Unknown: Oocytes that did not have their stage selected.
<img src="/images/o1.png" alt="" />	Discarded: Discarded oocytes are marked with a red cross.
<img src="/images/o2.png" alt="" />	Frozen: Frozen oocytes are marked with a snowflake symbol.
<img src="/images/thaw.PNG" alt="" width="55" height="38" />	Thawed: Thawed oocytes are marked with a melting snowflake symbol.
<img src="/images/o3.png" alt="" />	Selected for transfer: Pro-nucleus stages that are selected for an embryo transfer are marked with a green circle.
<img src="/images/group.png" alt="" width="55" height="38" />	Oocyte groups: If there is any colored bar on the left, the oocyte is part of an embryo / oocyte group.
<img src="/images/o4.png" alt="" />	Transferred ideal: Those that are to be transferred are marked with the ET (green) symbol.
<img src="/images/et.PNG" alt="" width="55" height="38" />	Transferred not ideal : Those that are to be transferred are marked with the ET (orange) symbol.
<img src="/images/o7.png" alt="" />	In arrest: Oocytes that stopped developing.
<img src="/images/Donation_Donated.png" alt="" width="55" height="38" />	Donation - Donated: Oocytes that have been donated are marked with an arrow right to the oocyte.
<img src="/images/Donation_received.png" alt="" width="55" height="38" />	Donation - Received: Oocytes that have been received from a donation are marked with an arrow left to the oocyte.
<img src="/images/o5.png" alt="" />	PBD: Oocytes that have been subjected to a polar body diagnosis.
<img src="/images/o10.png" alt="" width="55" height="38" />	PGD: Oocytes that have been subjected to a pre-implantation genetic diagnosis.
<img src="/images/o6.png" alt="" />	Assisted hatching: Embryos subjected to assisted hatching.
<img src="/images/o9.png" alt="" width="55" height="38" />	IVF: Indicates the insemination method used.
<img src="/images/o8.png" alt="" width="55" height="38" />	ICSI: Indicates the insemination method used.

Attention : if you switch back to the Table representation view, the detailed information about each oocyte will be lost.

Document the growth of oocytes/embryos from left to right. Always indicate the development stage first, then add a modification if needed.

<a href="/index.php?title=How_to_cultivate_oocytes_with_the_Graphical_representation_tab%3F">Click here</a> to read more about cultivating oocytes/embryos in the Graphical representation view.

Photo view

<img src="/images/Oo3.png" alt="" />	In Photo view mode, imported images are displayed when available. To import images, double-click the desired cell and click on Add file from the new window.
<img src="/images/Oo4.png" alt="" width="332" height="201" />	If Photo view is not active, a small camera icon will indicate that an image is available for viewing.

For both modes, you can view an enlarged image by moving your mouse over the cell.

Screenshot functionality

Click on the camera symbol to export the current representation into the <a href="/index.php?title=Notebook_/_screenshots">Notebook tool</a>. The following window will open:

If you do not close the notebook window and you take a second screenshot, it will automatically be added below the first one.

<a href="/index.php?title=Notebook_/_screenshots">Click here</a> to read more about the Notebook / screenshots tool.

Summed representation

An additional tab – Summed representation – provides a quick overview of the culture progress.

Data input is not possible here.

<a href="/index.php?title=MedITEX_IVF_how_to#Cycle">Click here</a> to read more about this section<a href="/index.php?title=MedITEX_IVF_how_to#Cycle"></a>.

<a href="/index.php?title=MedITEX_IVF_manual">Back to the MedITEX IVF menu</a>

<a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=MedITEX_IVF_manual"> </a><a href="/index.php?title=Cycles">Back to the cycles</a>

@@ Line 3: / Line 3: @@
 <tr>
 <td style="text-align: center;"><a href="/index.php?title=Cycle_Cryo"><img src="/images/plus48.png" alt="" width="48" height="48" /></a></td>
-<td><a href="/index.php?title=Cycle_Cryo">Cliccare qui</a>&nbsp;per Ciclo&nbsp;<strong>Cryo/Donation</strong> cycle.</td>
+<td><a href="/index.php?title=Cycle_Cryo">Cliccare qui</a>&nbsp;per <strong>Ciclo Solo Pick Up</strong> per <strong>Crioconservazione/Donazione</strong><strong>.&nbsp;</strong></td>
 </tr>
 </tbody>
@@ Line 17: / Line 17: @@
 </table>
 <p>&nbsp;</p>
-<p style="text-align: justify;">Every single oocyte/embryo can be documented in <strong>MedITEX IVF</strong>. The tab <strong>Culture </strong>allows  entries for a maximum of ten cultivation  days, although more than 5 to  6 days will most likely never be  necessary. In the overview, <strong>development stages</strong> and <strong>manipulations </strong>such as  <strong>insemination </strong>and <strong>injections</strong>, <strong>assisted hatching</strong>, <strong>polar body diagnosis</strong>, <strong> freezing </strong>and <strong>thawing processes</strong>, <strong>embryo transfer</strong> and <strong>more</strong>, can be  assigned to a specific date and stage.</p>
+<p style="text-align: justify;">Ogni singola cellula (ovocita/embrione) pu&ograve; essere documentata in&nbsp;<strong>MedITEX IVF</strong>. La tab&nbsp;<strong>Coltura </strong>permette di inserire informazioni per un massimo di 10 giorni di coltura, anche se nella maggior parte dei casi 5/6 giorni bastano. Nella overview &egrave; possibile assegnare per una specifica data e stadio della cellula informazioni riguradanti lo <strong>stadio di sviluppo</strong> e i&nbsp;<strong>trattamenti eseguiti</strong>, come per esempio l'<strong>inseminazione o iniezione</strong>,&nbsp;<strong>assisted hatching</strong>,&nbsp;<strong>diagnosi del globulo polare</strong>, <strong>crioconservazione&nbsp;</strong>e <strong>scongelamento</strong>, <strong>embrio transfer</strong>&nbsp;e <strong>molti altri</strong>.</p>
-<p>__TOC__</p>
 <p>&nbsp;</p>
-<p style="text-align: justify;">Before entering data, you should consider the focus      of you documentation.</p>
+<p style="text-align: justify;">Prima di cominciare ad inserire dati, sarebbe utile considerale lo scopo della documentazione.</p>
-<p style="text-align: justify;"><strong>MedITEX IVF</strong> provides two possibilities:</p>
+<p style="text-align: justify;"><strong>MedITEX IVF</strong> fornisce due possibilit&agrave;:</p>
 <ul>
-<li>A more simplified and summarized documentation, called <strong>Table representation</strong>; </li>
+<li>Una pi&ugrave; semplice e riassuntiva forma di documentazione, chiamata <strong>Rappresentazione Tabellare</strong>;</li>
-<li>And a more complete version &ndash; <strong>Graphical representation</strong>. </li>
+<li>e una pi&ugrave; completa, chiamata&nbsp;<strong>Rappresentazione Grafica</strong>. </li>
 </ul>
-<p style="text-align: justify;">The <strong>table </strong>and <strong>graphical representations</strong> are linked logically. Therefore, changes      made in one of the two views will also be represented in the other.</p>
+<p style="text-align: justify;">Le due rappresentazioni sono collegate in maniera logica, per cui cambiamenti riportati in una rappresentazione verranno automaticamente apportati anche all'altra.</p>
 <p style="text-align: justify;">&nbsp;</p>
 <table style="margin-left: auto; margin-right: auto;" border="0">
@@ Line 33: / Line 32: @@
 <td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
 <td>
-<p>You can only input data in one of the representations. If you are documenting oocytes/embryos in <strong>Table representation</strong>, <strong>Graphical representation</strong> will be locked.</p>
+<p>L'inserimento di dati &egrave; possibile solamente in una rappresentazione. Se si inizia a documentare informazioni nella <strong>rappresentazione tabellare,</strong> quella <strong>grafica </strong>verr&agrave; bloccata.</p>
-<p>You can lock/unlock these sections by clicking on the <strong><span style="color: #ff0000;">red </span></strong>or <span style="color: #339966;"><strong>green </strong></span>text at the bottom of this screen.</p>
+<p>Le due rappresentazioni possono essere bloccate/sbloccate cliccando sulla riga di testo&nbsp;<strong><span style="color: #ff0000;">rossa&nbsp;</span></strong>o&nbsp;<span style="color: #339966;"><strong>verde</strong><span style="color: #000000;"> sul fondo della schermo.</span></span></p>
+</td>
+</tr>
+</tbody>
+</table>
+<table border="0">
+<tbody>
+<tr>
+<td><img src="/images/datainput2.png" alt="" width="356" height="15" /></td>
+<td>
+<p>Sezione bloccata. L'input dei dati &egrave; possibile nell'altra rappresentazione.</p>
+<p>Nel nostro esempio questa scritta &egrave; presente nella rappresentazione grafica e indca che &egrave; attiva quella tabellare. Per attivare la rappresentazione grafica premere sulla riga rossa, che si trasformer&agrave; in verde.</p>
+</td>
+</tr>
+<tr>
+<td><img src="/images/datainput.png" alt="" width="207" height="15" /></td>
+<td>
+<p>Sezione sbloccata e l'input dei dati &egrave; permesso.</p>
+<p>Nel nostro esempio questa scritta &egrave; presente nella rappresentazone tabellare.</p>
+</td>
+</tr>
+</tbody>
+</table>
+<table border="0">
+<tbody>
+<tr>
+<td><img src="/images/hint48.png" alt="" width="48" height="48" /></td>
+<td>
+<p>Passando da Rappresentazione Grafica a Tabellare alcune informazioni (stadio embrioni) gi&agrave; inserite potrebbero andare perse.</p>
 </td>
 </tr>
@@ Line 40: / Line 67: @@
 </table>
 <p>&nbsp;</p>
-<h2>Treatments and treatment semen analysis</h2>
+<h2>Trattamenti e Spermiogrammi</h2>
 <table style="margin-left: auto; margin-right: auto;" border="0">
 <tbody>
@@ Line 55: / Line 82: @@
 <tr>
 <td style="text-align: left;"><a href="/index.php?title=Cryo thawing"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/CryoThawing.png" alt="" /></a></td>
-<td>Starts the thawing process.</td>
+<td>Scongelare campioni.</td>
 </tr>
 <tr>
 <td style="text-align: center;"><a href="/index.php?title=Cryo_storage"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/Cryopre.png" alt="" /></a></td>
 <td>
-<p style="text-align: justify;">Opens cryo storage so you can freeze oocytes/embryos.</p>
+<p style="text-align: justify;">Apre la criobanca permettendo la crioconservazione di ovociti/embrioni.</p>
 </td>
 </tr>
@@ Line 66: / Line 93: @@
 <td style="text-align: center;"><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/Reason.png" alt="" width="232" height="81" /></td>
 <td>
-<p>Documents the reason why there was no embryo transfer for this cycle.<strong>&nbsp;</strong></p>
+<p>Campo riservato alla documentazione delle ragioni di mancato transfer.</p>
-<p>You can edit the options available here at the <a href="/index.php?title=MedITEX_IVF_Settings#System_tables">system tables</a> in the settings.<br /><br /></p>
 </td>
 </tr>
@@ Line 73: / Line 99: @@
 <td style="text-align: center;"><a href="/index.php?title=Treatments and treatment semen analysis"><img style="display: block; margin-left: auto; margin-right: auto; border: 2px dotted blue;" src="/images/cryoprov2.png" alt="" width="400" height="72" /></a></td>
 <td>
-<p><strong>Treatments and semen analysis</strong>:</p>
+<p><strong>Trattamenti e spermiogrammi:</strong></p>
-<p style="text-align: justify;"><strong>Double-click</strong> to see <strong>treatment and semen analysis</strong>. You do not need a <strong>semen analysis</strong> for <strong>thawed cycles</strong>.</p>
+<p style="text-align: justify;"><strong>Doppio-click</strong>&nbsp;per visualizzare i <strong>trattamenti e gli spermiogrammi collegati alla terapia</strong>. Per <strong>cicli da scongelamento</strong> di zigoti/embrioni non &egrave; necessario collegare uno spermiogramma</p>
-<br /></td>
+</td>
-</tr>
-<tr>
-<td style="text-align: center;"><img src="/images/datainput2.png" alt="" width="356" height="15" /></td>
-<td>This indicates that either the <strong>Table </strong>or <strong>Graphical representation</strong> are locked and cannot be changed. Click the text to unlock.</td>
-</tr>
-<tr>
-<td style="text-align: center;"><img src="/images/datainput.png" alt="" width="207" height="15" /></td>
-<td>Indicates that this section is unlocked and can be edited.<br /></td>
 </tr>
 </tbody>
 </table>
 <p>&nbsp;</p>
-<h2 style="text-align: left;">Table representation</h2>
+<h2 style="text-align: left;">Rappresentazione tabellare</h2>
-<p>This table allows you to <strong>efficiently and quickly</strong> document all types of cycles: <strong>aspiration </strong>(IVF/ICSI), <strong>thawing</strong>, etc.</p>
+<p>Questa rappresentazione pernette di documentare in maniera <strong>efficiente e veloce </strong>tutti i tipi di ciclo: <strong>Con Pick Up&nbsp;</strong>(FIVET/ICSI), <strong>Scongelamento</strong>, etc.</p>
 <table style="margin-left: auto; margin-right: auto;" border="0">
 <tbody>
@@ Line 98: / Line 116: @@
 <td><img style="display: block; margin-left: auto; margin-right: auto;" src="/images/hint48.png" alt="" width="48" height="48" /></td>
 <td>
-<p><strong>Advanta</strong><strong>ges</strong>: faster and simpler.</p>
+<p><strong>Vantaggi</strong>: veloce e semplice.</p>
-<p><strong>Disadvantages</strong>: documentation of sums only, not on a cell level.</p>
+<p><strong>Svantaggi</strong>: Documentazione sommaria, non a livello di singola cellula.</p>
 </td>
 </tr>
@@ Line 105: / Line 123: @@
 </table>
 <p>&nbsp;</p>
-<h3 style="text-align: justify;">Options for cryopreservation</h3>
+<h3 style="text-align: justify;">Opzioni per crioconservazione</h3>
-<p style="text-align: justify;">From the <strong>Culture tab</strong>, you have three options for freezing oocytes/embryos:</p>
+<p style="text-align: justify;">Nella tab della <strong>Coltura</strong>, ci sono tre opzioni per la crioconservazione di ovociti/embrioni:</p>
 <table style="margin-left: auto; margin-right: auto;" border="0">
 <tbody>
@@ Line 112: / Line 130: @@
 <td style="text-align: center;"><img src="/images/ov2.png" alt="" width="450" height="272" /></td>
 <td style="text-align: justify;">
-<p><strong>1. </strong>If you enter the <strong>number of oocytes</strong>, <strong>PNs</strong> or <strong>embryos</strong>, the program will <strong>automatically transfer it</strong> to the <strong>correct field</strong> of the <strong>cryo preservation</strong> window.</p>
+<p><strong>1. </strong>Inserendo il numero si <strong>ovociti, PN o embrioni</strong>&nbsp;il programma inserir&agrave; <strong>automaticamente</strong> il numero nella <strong>sezione corretta</strong> nella finestra di <strong>crioconservazione</strong>.</p>
 </td>
 </tr>
 <tr>
 <td style="text-align: center;"><a href="/index.php?title=Cryo_storage"><img style="border: 2px dotted blue;" src="/images/cryocon.png" alt="" /></a><br /></td>
-<td style="text-align: justify;"><strong>2. </strong>You can also open the <a href="/index.php?title=Cryo_storage"><strong>cryo storage</strong></a>, if you <strong>double-click </strong>the number of oocytes for <strong>Cryo</strong> in the <strong>table view</strong>.</td>
+<td style="text-align: justify;"><strong>2. </strong>Facendo doppio-click sul numero di cellule crioconservate dalla rappresentazione tabellare , &egrave; possibile aprire la criobanca.&nbsp;<a href="/index.php?title=Cryo_storage"><strong>cryo storage</strong></a></td>
 </tr>
 <tr>